ABSTRACT
Abstract: Purpose: To investigate the microbiological, inflammatory and oxidant effects of adjuvant ozone administration in experimental rat vascular graft infection model which has not been previously investigated. Methods: Forty adult Wistar rats were divided into Sham, Control, Vancomycin, Ozone, Vancomycin+Ozone groups. Grafts were inoculated with Methicillin-resistant Staphylococcus aureus (MRSA) strain and implanted subcutaneously. Rats were treated intraperitoneally with ozone and /or intramuscularly with vancomycin for 10 days. Grafts were evaluated by quantitative bacterial cultures. Blood samples were harvested for determination of thiol-disulphide and cytokine profiles. Results: There was no significant difference in bacterial counts between Control and Ozone Groups. In the Ozone Group median colony count was significantly higher than the Vancomycin and Vancomycin+Ozone Groups. Total thiol and disulphide levels increased and disulphide/native thiol and disulphide/total thiol ratios decreased in Ozone Group significantly. Albumin levels decreased significantly in Vancomycin and Vancomycin+Ozone Groups compared to the Sham Group. IL-1 and TNF-alpha levels significantly increased in infected rats. Decreased levels of VEGF due to infection reversed by ozone therapy in control and vancomycin groups. Conclusions: We didn't observe any benefit of the agent on MRSA elimination in our model. Likewise, effects of ozone on thiol-disulphide homeostasis and inflammatory cytokines were contradictory.
Subject(s)
Animals , Male , Oxidants, Photochemical/pharmacology , Ozone/pharmacology , Staphylococcal Infections/drug therapy , Disulfides/blood , Methicillin-Resistant Staphylococcus aureus/drug effects , Vascular Grafting , Reference Values , Time Factors , Vascular Diseases/microbiology , Serum Albumin/analysis , Vancomycin/pharmacology , Colony Count, Microbial , Random Allocation , Reproducibility of Results , Cytokines/blood , Treatment Outcome , Rats, Wistar , Transplants/microbiology , Methicillin-Resistant Staphylococcus aureus/growth & development , Homeostasis/drug effects , Anti-Bacterial Agents/pharmacologyABSTRACT
En este trabajo son discutidos los aspectos tomográficos observados en ocho pacientes con invasión vascular por Aspergillus, siete de ellos presentando leucemia como enfermedad de base y uno linfoma no-Hodgkin. Cuatro de los pacientes se encontraban en fase inicial de la enfermedad y presentaban nódulos de contorno irregular y mal definidos, con el signo del halo y sin excavación. Dos en fase de resolución, mostraban nódulos excavados, con contornos internos irregulares y contenido heterogéneo, reticulado. En uno de los pacientes el aspecto era de consolidación con necrosis y excavación, y el último presentaba nódulo intracavitario, con el signo de creciente aéreo. En seis de los pacientes la lesión estaba en contacto con la superficie pleural. La tomografía computada contribuye de forma importante tanto en el diagnóstico de la invasión vascular por aspergillus, como también en el seguimiento de la evolución de la enfermedad.